To observe the effect of Murraya Paniculat leaf on volumetric bone mineral density, blood calcium, blood phosphorus, Alkaline phosphatase, bone histomorphology in ovari-ectomized rats. Methods:A total of 60 SPF SD female rats were taken as models by removing bilateral ovaries. After 7 days of operation, all rats were divided into six groups：model group (normal saline, 0.4 mg/kg·d), control operation group (normal saline, 0.4 mg/kg·d), Estradiol Valerate group(0.4 mg/kg·d), Murraya Paniculat leaf high dose group (200 mg/kg·d), Murraya Paniculat leaf middle dose group (100 mg/kg·d) and Murraya Paniculat leaf low dose group (50 mg/kg·d). Drugs were given once a day for 90 days. Using electrode analysis to measure volumetric bone mineral density of rats and velocity method to measure blood calcium and blood phosphorus and alkaline phosphatase. Use HE staining on the right side of the femur of rats, then measured the average width and trabecular bone area percentage. Results:Compared with the control group, volumetric bone mineral density of model group rats were reduced (P<0.05), blood phosphorus and Alkaline phosphatase of model group rats were lower, and the difference is significant (P<0.05), also, the average width and trabecular bone area percentage of model group rats were lower (P<0.05). No difference was found in blood phosphorus. Compared with model group, volumetric bone mineral density and blood calcium and the average width and trabecular bone area percentage were higher in Murraya Paniculat leaf high dose group (P<0.05). No difference was found in blood phosphorus. Also, alkaline phosphatase in Murraya Paniculat leaf low dose group was lower with significant difference (P<0.05). Conclusion:Murraya Paniculat leaf could influence volumetric bone mineral density, blood calcium, blood phosphorus, alkaline phosphatase, the average width and trabecular bone area percentage in ovari-ectomized rats. It has anti-osteoporosis effect.