To optimize the matrix formulation of the Guihong Huoxue gel paste.Methods:The effects of various factors on the properties of matrix were investigated by single factor investigation.The three factors with largest effects were selected as the study factors of central composite design.The optimal prescription was predicted by the comprehensive score，and would be verified by clinical practice.Results:Guihong Huoxue analgesic gel paste matrix optimum of ratio was：NP-700 2.38 g，PVP-K90 2.25 g，aluminium glycinate 1 g，tartaric acid 0.15 g，EDTA 0.025 g，kaolin 2 g，glycerin 19 g，propylene glycol 8 g，CMC-Na-water extract of safflower 43 g，ointment 10 g.Conclusion:The Guihong Huoxue of the optimal preparation matrix formula of analgesic gel paste looks smooth，moderate viscosity，good skin following and good performances.