To determine the contents of three tanshinones in Yangxue Zhiteng Pill by QAMS and to validate its feasibility.Methods:The tanshinone IIA as the internal reference substance，the relative correction factor of tanshinone I and cryptotanshinone were determined by HPLC on different instruments and chromatographic columns.The content of tanshinone IIA in Yangxue Zhiteng Pill was authentically determined by the external standard method，and the content of tanshinone I and cryptotanshinone was calculated by external standard method and relative correction factor respectively.Results:The QAMS could determine the content of tanshinone I，cryptotanshinone and tanshinone IIA.No significant differences between the quantitative results of QAMS and external standard method were observed.Conclusion:The QAMS can determine the quality of content of tanshinone in Yangxue Zhiteng Pill.