To study of activin A and E selectin in the pathogenesis of “shanghuo” people and influence effect through the determination of serum content and the level of gene expression in peripheral blood mononuclear cells of activin A or E-selectin who suffered from excessive internal heat. Methods:Clinical lit crowd and gentleness in blood samples was collected and serum and peripheral blood mononuclear cells from blood samples was extracted. By enzyme-linked immune sorbent adsorption assay, the content of serum activin A and E-selectin were determined, and RT-PCR detection was sued to detect gene expression level of activin A and E-selectin. Results:The level of activin A and E-selectin in serum of patients with excessive internal heat was higher than that of normal and healthy people. The level of activin A and E-selectin gene expression in peripheral blood mononuclear cells of patients with internal heat was higher than that of normal and healthy people. The gene expression level of activin A and E-selectin in peripheral blood was higher than that of yin deficiency and heat syndrome. Conclusion:Shanghuo is a local inflammation. Under stress, activin A levels are elevated, effecting inflammatory mediators or itself as a mediator of inflammation activated vascular endothelial cells stimulate E-selectin secretion and synthesis, induced inflammatory reaction and to cause the disorder of lipid metabolism in the body's physiological balance, causing Shanghuo.