To do research on the regulating effect of 5-HT1B receptors in the migraine rat model of electrical stimulating dura mater via electroacupuncture.Methods:40 male SD rats were divide into 5 groups, 8 for each group.Except control group, the electrical stimulation of the dura surrounding the superior sagittal sinus was carried out at the 1st, 3rd and 5th day.The control group received electrode implantation surgery only.At the 2nd, 4th and 6th day, single acupoint group and double acupoint group were received electroacupuncture as treatment.Sham acupuncture group received acupuncture at a non-acupuncture point following dural stimulation.The facial mechanical withdrawal threshold was taken at the 2nd, 4th and 6th day following dural stimulation.Eight rats from each group were decapitated, and the TG and TNC were removed and detected 5-HT1B receptor mRNA expression by real-time quantitative PCR.5-HT1B receptor protein was evaluated by western blot.Results:Compared with the control group, the mechanical withdrawal threshold of model group was declined significantly (P<0.05).Compared with the model group, the mechanical withdrawal threshold of single acupoint group and double acupoint group was declined significantly (P<0.05).Compared with the model group, 5-HT1B receptor mRNA and protein in TNC and TG tissue of rats in single control group was declined significantly (P<0.05).Compared with the model group, 5-HT1B receptor mRNA and protein in TNC and TG tissue of rats in single acupoint group and double acupoint group was increased significantly (P<0.05).Conclusion:The electroacupuncture could significantly improve the damage of rat dura mater of superior sagittal sinus in following electrical stimulation by releasing neurotransmitter, which may be the mechanisms of migraine treatment.