To investigate the effects of Longmu Zhuanggu Granule on glucocorticoid-induced osteoporosis rats. Methods:Rat osteoporosis models were prepared with low calcium feed and muscle injection with hydride prednisone. Compound group, Chinese medicine group, chemical medicine group, and model group of rats were given Longmu Zhuanggu Granule, its Chinese medicine, its chemical medicine, and distilled water respectively by intragastric administration. Normal control rats were feed with normal fodder. Related indicators were detected after 35 days of administration. Results:Indicators of the compound group of rats, such as body weight, thymus index, calcium and phosphorus in blood, femoral weight, bone calcium, femoral epiphysis end diameter of the cortex, bone mineral density, the density of backbone medulla, cortical density, bone mineral density, etc, were significantly superior to the model group. Urinary calcium of the compound group of rats was significantly lower than that of the model group. Conclusion:Longmu Zhuanggu Granule possesses a well therapeutic effect on glucocorticoid-induced osteoporosis.