To observe the protective effects of Yinxing Mihuan Oral Solution (YXMH) on acute myocardial ischemia injury of dogs. Methods: A total of 36 dogs were randomly divided into model group, diltiazem group (5.0 mg/kg), Gastrodia Tuder Halimasch Powder group(100 mg/kg), Gingkgo biloba leaf extract group (3 mg/kg), low and high doses of YXHM (103 mg/kg; 206 mg/kg). The model of acute myocardial infarction was established by ligating anterior descending coronary arteries of dogs. The changes of coronary arteries blood flow volume, blood oxygen content, myocardial consumption of oxygen, myocardial oxygen utilization and myocardial infarct size were observed at different points of time after myocardial ischemia. Results: Compared with the model group, YXHM with low and high doses groups, Gastrodia Tuder Halimasch Powder group and Gingkgo biloba leaf extract group could significantly reduce myocardial infarct size (P<0.05); Meanwhile, YXHM with high dose could decrease venous oxygen content 30 minutes after myocardial ischemia and increase myocardial consumption of oxygen and myocardial oxygen utilization after 15minutes, 30minutes and 60 minutes after myocardial ischemia (P<0.05). YXHM with high dose also increased coronary blood flow from 60 minutes to 180 minutes after myocardial ischemia (P<0.05). Conclusion: YXHM can facilitate myocardial consumption of oxygen, increase coronary blood flow and mycardial oxygen supply, improve myocardial oxygen utilization. Pharmaceutical effect of YXHM is better than Gingkgo biloba leaf extract and Gastrodia Tuder Halimasch Powder.