To evaluate the protective effects of Yinxing Mihuan Oral Solution on brain tissues of ischemia/reperfusion injury model rats, and to explore the pharmaceutical effect in protecting injured brain tissue from neurological deficit score, cerebral infarction range and anti-oxidant effect. Methods: Rats ischemia/reperfusion model was established by nylon thread insertion into middle cerebral artery, and then the model rats were randomized into 8 groups of sham operation group, model group, ginaton group, ginkgo leaf extraction group, gastrodia tuder halimasch tablet group, gastrodia tuder halimasch powder group, Yinxing Mihuan Oral Solution 618 mg/kg group and Yinxing Mihuan Oral Solution 309 mg/kg group. The sham group was only subjected to carotid artery separation without occlusion. Rats in other groups was blocked middle cerebral artery by a thread plug, and reperfusion after 1.5 h, resulting in ischemia-reperfusion injury model. At the same time, drugs were administrated from duodenum with a dose of 3 mL/kg respectively. Sham group was given saline at the same volume instead. Neurological deficit scores were taken one-side blinded 6 h and 24 h after ischemia onset. After the second time of scoring, the rats were sacrificed, and blood sample were taken for serum level examination of SOD, MDA and GSH-PX. Brain was taken for TTC staining to calculate cerebral infarction range. Results: Yinxing Mihuan Oral Solution groups had a significant reduce in cerebral infarction area and MDA level of serum, while a significant increase in 24 h neurological deficit score and serum level of SOD and GSH-PX compared with model group, which was also better than the results of ginkgo leaf extraction group and gastrodia tuder halimasch group. Conclusion: Brain infarction area due to ischemia/reperfusion of rats was reduced by Yinxing Mihuan Oral Solution, with increased neurological deficit scores. The results suggest the brain protective efficacy of Yinxing Mihuan Oral Solution might be related to its anti-oxidative effect.