To investigate the effects of Huoxue Bushen Decoction in the treatment of anovulatory infertility and its effect on ovulation.Methods:A total of 96 cases of patients with anovulatory infertility treated in Yan'an traditional Chinese medicine hospital from June 2015 to December 2016 were randomly divided into observation group (n＝48) and control group (n＝48) according to random number table. The control group was given Clomifene Citrate Capsules at 5th day of menstrual cycle, with continuous use of 5d; the observation group was added Huoxue Bushen Decoction. Two groups of patients were treated for 3 menstrual cycles. The therapeutic effects of two groups were compared before and after treatment. The changes of serum hormone levels, the diameter of dominant follicles and the thickness of endometrium were observed before and after treatment. Results:The total efficiency of the observation group (89.58%) was higher than the control group (70.83%) (P<0.05); The serum level of E2 was increased after treatment in two groups (observation group t=24.739, control group t=16.732, P<0.05), and the levels of LH and FSH were decreased (observation group t=27.483,12.144, control group t=14.952, 5.380, P<0.05); the serum level of E2 after treatment in observation group was higher than the control group (t=9.276, P<0.05), and LH, FSH levels were lower than the control group (t=17.077, 7.456, P<0.05); the dominant follicle diameter and endometrial thickness of two groups after treatment were increased (observation group t=19.566, 10.438, control group t=15.348, 6.103, P<0.05); the dominant follicle diameter and endometrial thickness of the observation group after treatment was larger than the control group (t=4.856, 4.762, P<0.05); the successful ovulation rate of the observation group (83.33%) was higher than that of the control group (64.58%) (P<0.05). Conclusion:Huoxue Bushen Decoction is effective in the treatment of patients with anovulatory infertility, and can significantly improve the ovulation rate.