To observe the changes of monoamine neurotransmitters and cAMP, cGMP of liver depression model rats caused by chronic immobilization stress, and to study the mechanism of Albiflorin and Paeoniflorin and material basis of “emolliating liver to resolve depression”.Methods:The model was induced by chronic immobilization stress and solitary.HPLC-ECD was used to analyze the levels of monoamine neurotransmitters.The contents of cAMP and cGMP were detected by ELISA.The changes of the model group were observed before and after administration.Results:Compared with normal group，the levels of monoamine neurotransmitters, noradrenaline, dopamine and 5-hydroxyindoleacetic acid in the model rats decreased significantly (P<0.001 or P<0.01).The content of cAMP decreased, while the content of cGMP increased (P<0.01 or P<0.01); Compared with model group, the high dose of Albiflorin and Paeoniflorin can increase the levels of monoamine neurotransmitters significantly (P<0.05).The high dose of Paeoniflorin can increase the content of cAMP (P<0.05), while the content of cGMP were decreased significantly in high dose of Albiflorin and low dose of Paeoniflorin (P<0.05).Conclusion:Albiflorin and Paeoniflorin as the main ingredient of Radix Paeoniae Alba, may resist the state of liver depression through the regulation of cAMP, cGMP and monoamine neurotransmitters in brain, so as to play the role of emolliating liver to resolve depression.