To observe the effects of Tonifying marrow moxibustion on learning and memory ability, expression of 8-OHdG and T-AOC in serum and cerebral and nephridial tissues in D-galactose induced aging mice, and to explore the mechanism by comparing points of the two groups (ST36 and GB39, DU20 and RN4) in the effect of delaying aging. Methods:A total of 75 SPF Kunming male mice (3 months old, weight：30±2g) were randomly and equally divided into the normal physiology group, model group, moxibustion 1 group ( ST36 and GB39), moxibustion 2 group ( DU20 and RN4) and moxibustion 3 group ( non-acupuncture) after Morris Water Maze Test. Mice in treatment and model groups were injected subcutaneously back of necks by 1000 mg/kg/day of D-galactose for 56 days, while mice in the normal physiology group were injected the same dosage of normal sodium in the same way for 56 days. Mice in the treatment groups were treated with moxibustion with 3 segments /point/time, unilateral or two points (altogether 6 segments) every day from the 13th day after molding. Mice in the normal physiology group and model group were not treated with therapeutic intervene, but to give catching stimulation for the same time and degree as the treatment groups. After modeling and treatment, all rats were tested by Morris Water Maze Test. The eyeball blood was taken, and cerebral tissue was collected by beheading. And kidney was taken by laparotomy to prepare the cerebral and kidney tissue homogenate. Expression of 8-OHdG and T-AOC in serum and the cerebral and kidney tissues were detected by ELISA method. Results:1) The Morris Water Maze results showed that, before modeling and treatment, there were no difference of learning and memory among the model and the other four groups in the indicators of Morris Water Maze Test (P>0.05); There were no significant differences among the five groups after modeling and treatment (P<0.05). Comparison between any two means showed that, 1）After modeling and treatment, there were significant difference compared with modeling group, normal physiology group, moxibustion 1 group and moxibustion 2 group, the indexes of “incubation, platform quadrant ratio, standing time, seeking time, the percentage of the original platform and crossing platform time” all had significant differences; 2）Compared with the physiology group and the moxibustion 3 group, there was significant difference (P<0.05); Compared with the moxibustion 3 group and the moxibustion 1 and moxibustion 2 group there was significant difference (P<0.05). 2) The ELISA results of the serum and cerebral-kidney tissue showed：1）the contents of 8-OHdG in the serum and cerebral-kidney tissue of model group were higher than that of the physiology group, moxibustion 1 group, moxibustion 2 group and moxibustion 3 group, which had statistic differences (P<0.05); compared with physiology group and the moxibustion 3 group, there was significant difference (P<0.05 ). 2）The contents of T-AOC in the serum and cerebral-kidney tissue of model group were significantly lower than those in the physiology group, compared with moxibustion 1 group, moxibustion 2 group and moxibustion 3 group, which had statistic differences (P<0.05); compared with the physiology group and moxibustion 3 group, there was significant difference (P<0.05); there were significant differences compared moxibustion 1 group and moxibustion 2 group with moxibustion 3 group (P<0.05). Conclusion:The mechanism of tonifying marrow moxibustion in preventing brain aging may be related to decreasing the expression of 8-OHdG which induced by D-galactose, inhibiting the damage cell DNA, increasing the content of T-AOC, and improve function of the anti-oxidative stress injury.