To explore the effects of Kangshuai Decoction combined with hormone replacement therapy on the endocrine and ovarian function in the treatment of women with premature ovarian failure (POF). Methods:A total of 500 cases of POF patients treated in our hospital from January 2014 to October 2016 were selected and randomly divided into observation group and control group, with 250 cases in each group. Control group was treated with hormone replacement therapy, and the observation group was treated with Kangshuai Decotion on the basic of control group. 21 days were for a treatment course. Both groups were treated for 3 courses continuously. The clinical effects of 2 groups were statisticsed, and the endocrine hormone levels, ovarian function of 2 groups were compared before and after treatment; the incidence of adverse reactions of 2 groups in the treatment were recorded. Results:The total effective rate of observation group after treatment was 92.40%, which was significantly higher than the .80.80% of control group (P<0.05); the levels of serum E2 in 2 groups after treatment were significantly higher than before treatment, and observation group was significantly higher than control group (P<0.01); the levels of serum LH and FSH in 2 groups after treatment were significantly lower than before treatment, and observation group was significantly lower than control group (P<0.05 or P<0.01); compared with control group, the OV, FN, endometrium thickness of 2 groups increased, and the PSV of 2 groups quickened after treatment. The observation group was significantly better than control group (P<0.01); the incidence of adverse reactions in the treatment group (22.00%) was significantly lower than that of the control group (3.8.00%) (P<0.05). Conclusion:Kangshuai Decoction combined with hormone replacement therapy in the treatment of POF can effectively regulate the level of endocrine hormones in women, and significantly improve their ovarian function. The clinical efficacy is superior to simple hormone replacement therapy.