To investigate the distribution of serum lipids, serum bilirubin and serum proteins on six-qi, in order to provide a basis for the prevention and treatment of chronic diseases in the community. Methods:According to the data of 3 years' physical examination of the Shuangyushu community health service center, it was divided into 2 groups of men and women by sex and then 6 groups according to six-qi respectively. The comparative study of biochemical indexes was carried out and the related data were statistically analyzed. Results:The average level of TC, HDL and LDL was higher in the one-qi and six-qi (winter and spring) average, and the lowest in three-qi or four-qi (summer and autumn); the curve of TG was the opposite. The mean of TBil, DBil, IBil, TP and ALB reached peak value before and after two-qi (spring). Conclusion:Blood lipid, serum bilirubin and serum protein level were correlated with six qi.