To explore the effects of Chinese herbal medicine enema combined with external electric field thermotherapy in vitro on inflammatory factors, immune function and hemorrheology in patients with chronic pelvic inflammatory disease (CPID). Methods:A total of 500 cases of CPID patients treated in our hospital were randomly divided into observation group and control group, with 250 cases in each group. All patients were given conventional treatment, patients in control group were treated with external field thermotherapy, patients in observation group were treated with Chinese medicine enema combined with external field thermotherapy in vitro. All the patients were treated for 30 days continuously. The clinical effects of 2 groups were statistically analyzed, and the inflammatory factors, immune function and hemorrheology of 2 groups were compared before and after the treatment. Results:The total effective rate of observation group after treatment was 94.40%, which was significantly higher than 81.60% of control group (P<0.05); the serum IL-2 levels in 2 groups after treatment increased significantly compared with that before treatment, and observation group was significantly higher than control group (P<0.01); the serum IL-6, CRP and TNF-α levels of 2 groups after treatment significantly decreased compared with before treatment, and levels of observation group were significantly lower than those in control group (P<0.01); the CD4+ cell proportion and CD4+/CD8+ ratio of 2 groups after treatment increased significantly compared with before treatment (P<0.01), and the levels in the observation group were significantly higher than those in control group (P<0.05 or P<0.01); the high/low shear whole blood viscosity, plasma viscosity, erythrocyte aggregation index and the fibrinogen levels of 2 groups after treatment decreased significantly, and the levels of observation group were significantly lower than those in control group (P<0.01). Conclusion:Chinese herbal medicine enema combined with external field hyperthermia in the treatment of CPID can effectively reduce the level of serum inflammatory factors, regulate its immune function to normal, and significantly improve its hemorrheology, thereby enhancing the clinical efficacy.