To investigate better administration method of compatibility of Flos Puerariae Lobatae and Semen Hoveniae, and the influence on content of striatum dopamine (DA), 5-hydroxytryptamine (5-HT) and activity of hippocampus acetylcholinesterase (AchE). Methods:ICR mice were randomly divided into eight groups, including blank control group, model group, positive control group, Gehua group, Zhijuzi group, and combination group 1, 2, 3. The mouse model of acute alcoholism was established by giving alcohol to mice orally after 30 min for taking preventive administration method. The striatum DA, 5-HT and hippocampus AchE were measured after 2 h. Results:The compatibility of Flos Puerariae Lobatae and Semen Hoveniae was superior by prevention to treatment. The contents of striatum 5-HT and activity of achippocampus AchE in the model group were increased compared with those in the blank control group (P<0.05). The contents of DA were obviously reduced in Zhijuzi groups (P<0.05). The contents of 5-HT were significantly decrease in combination group 1, 3 (P<0.05). Conclusion:There is an improvement on neurobehavioral abnormality by regulating the contents of DA, 5-HT and activity of AchE of compatibility of Flos Puerariae Lobatae and Semen Hoveniae.