To explore the effect of the burn wound healing in rats with diabetes by the combined treatment of estrogen and compound Radix Arnebiae Oil. Methods:A total of 40 male SD rats were divided randomly into 5 groups, inclduing blank group, model group, estrogen group, Compound Radix Arnebiae Oil group, combined estrogen and Compound Radix Arnebiae Oil group. Except the blank group, the others were established the diabetes model. After the success of the model building, the whole rats were caused deepⅡdegree burn in the back. Apart from the blank group and the model group, each treatment group was treated by the appropriate treatment methods. The wound healing rate and blood glucose of each rat was observed, and the expression of TGF-β1and VEGF in 1, 8, 15, 22 days real-time were detected. The wound tissue pathology of each rat was observed and made pathological grades. Results:Compared with the blank group, rat wound healing rate of the model group was significantly reduced, and the blood sugar level increased significantly. The level of TGF-β1and VEGF decreased significantly, and the pathological grades rise significantly (P<0.05). Compared with the model group, the rat wound healing rate of each treatment group was obviously higher, and the blood sugar level was significantly lower. The TGF-β1and VEGF were significantly higher, and the pathological score significantly reduced (P<0.05). Conclusion:The estrogen and the Compound Radix Arnebiae Oil can promote the healing of burn wound on diabetic rats and play a role of collaborative work.