To explore the proliferation inhibition effect of curcumin on human pancreatic cancer SW1990 cell and the influence on Wnt signaling pathway. Methods:The human pancreatic cancer cells SW1990 in experimental-L/M/H group were treated with final concentration of 20, 50, 100 μmol/L curcumin, 100 μL/hole, and cells in control group were treated with isovolumetric saline. Each group was intervened for 24, 48, 72 h respectively. The proliferation and apoptosis were detected by methyl thiazolyl tetrazolium (MTT) method and Annexin-V/FITC double staining respectively, expression of β-Catenin mRNA and β-Catenin mRNA protein in human pancreatic cancer SW1990 cell were detected by reverse transcription PCR (RT-PCR) method and cell climbing immunocytochemistry technique respectively. Results:72 h after intervened, the proliferation inhibition rates (%) of human pancreatic cancer SW1990 cell in experimental-L/M/H group were 4.59±1.32, 40.01±3.87, 60.98±5.97 respectively; Apoptosis rates (%) in control group and experimental-L/M/H group were 8.14±1.25, 13.04±1.03, 27.15±1.62, 59.21±1.46 respectively; β-Catenin protein immunocytochemistry (ICC) score (score) were 5.78±0.56, 4.42±0.61, 2.57±0.58, 1.38±0.63 respectively. The apoptosis rate in experimental groups were higher than that in control, and β-Catenin mRNA relative expression and β-Catenin protein ICC score were lower than those in control group (P<0.05), and the proliferation inhibition rate, apoptosis rate in experimental groups increased gradually with the increase of curcumin action concentration (P<0.05). Proliferation inhibition also increased gradually with the lengthen of action time (P<0.05); the β-Catenin mRNA relative expression and β-Catenin protein ICC score decreased gradually with the increase of curcumin action concentration (P<0.05). Conclusion:Curcumin can down-regulate the expression of β-Catenin in human pancreatic cancer SW1990 cell effectively, inhibit the Wnt signaling pathway excitation, and has significant proliferation inhibition effect, which has an obvious time-dose dependence.