To explore the academic experience of Professor Qin Liangfu's treatment from spleen, and to observe the clinical efficacy of acupuncture from spleen on central obesity and hyperlipidemia by randomized controlled study, and to the possible mechanism of improving insulin resistance. Methods:A total of 60 patients were randomly divided into acupuncture group and control group. Qin Liangfu's treatment from spleen was used in the acupuncture group, and abdominal three acupoints Tianshu (ST 25), Zhongwan (RN 12), Guanyuan (RN 4) with lower confluent acupoints of Zusanli (ST 36), Shangjuxu (ST 37), Xiajuxu (ST 39) were selected. The control group was treated with routine diet control. Body mass index (BMI), waist hip ratio, blood cholesterol (TC), triglyceride (TG), serum low density lipoprotein (LDL-C), serum high density lipoprotein (HDL-C) and the difference of fasting blood glucose (FBG), fasting insulin (INS) and tumor necrosis factor alpha (TNF-alpha), Adiponection, Ghrelin, insulin resistance (ISI) were choosed to observe the effect and the possible mechanism of acupuncture on central obesity and hyperlipidemia. Results:There was no significant difference in BMI, waist hip ratio, blood TC, blood TG, blood LDL-C, blood HDL-C, FBG, INS, TNF-alpha, Adiponection, Ghrelin and ISI before treatment. For patients in acupuncture group with treatment from the spleen, BMI (P<0.05), waist hip ratio (P<0.01), TC, TG (P all<0.01) and LDL-C content decreased (P<0.05), and the content of HDL-C increased (P<0.01); there was no significant difference in the control group. Compared with the control group, BMI, waist hip ratio decreased and HDL-C increased (P all<0.01) with significant difference. There was significant difference in FBG, INS, ISI (P all<0.05), Adiponection (P<0.01), Ghrelin (P<0.05) in the acupuncture group. The total effective rate in the control group was 50%, while 80% in the acupuncture group. There was significant difference between two groups (P<0.01). Conclusion:Acupuncture treatment from the spleen could improve the lipid metabolism and body index of the patients with central obesity and hyperlipidemia, and the possible mechanism may be related with improving insulin resistance and increasing insulin sensitivity.