To investigate the clinical curative effect of the acupuncture based on midnight-noon cycle of qi in combination with interferon on cervical cancer co-infection of human papilloma virus (HPV), and to observe the effects on Th17 cytokines. Methods:A total of 70 cases of patients with cervical cancer co-infection of HPV in our hospital included into the study, and were randomly divided into the control group and the observation group, with 35 cases in each group. Two groups of patients accepted chemotherapy regimens of paclitaxel + carboplatin and recombinant human interferon α-2βevenly cervical surface, and the observation group combined with acupuncture based on midnight-noon cycle of qi on the basis of the above treatment. The clinical curative effect, Karnofsky Performance Status (KPS), peripheral blood Th17 cells percentage changes of two groups were compared, and the adverse reactions were observed. Results:1)KPS scores improved in the two groups after treatment, and the increase trend of observation group was more obviously than the control group. The differences were statistically significant (P<0.05). 2)The clinical total effective rate of observation group was 74.29%, and that was 42.86% in the control group. The differences were with statistically significant (χ2=3.29, P<0.05). After treatment, 26 cases of HPV turned to negative in the observation group, and the negative conversion ratio was 74.29%,.12 cases turned to negative in the control group, and the negative conversion ratio was 34.29%. The differences were statistically significant (χ2=2.83, P<0.05). 3)After a stage of treatment, peripheral blood Th17 percentage in the two groups decreased, and the observation group decreased more obvious than the control group. The differences were statistically significant (P<0.05). Conclusion:The acupuncture based on midnight-noon cycle of qi can improve the immunosuppression of cervical cancer co-infection of HPV, and can significantly improve the patient's quality of life.