To conduct a quantitative study on the modeling factors of animal model of cholesterol gallstone induced by stone feed to provide references for improving the efficiency of modeling and evaluating the effectiveness of drugs. Methods:Literatures on animal models of cholesterol gallstone induced by stone feed published during 1993 to 2016 were collected, and the important information such as the animal species, the feed formulation, the rate of stone formation, the test indexes and the drugs were input. A quantitative statistics as well as an analysis of modeling elements were conducted. Results:The commonly used animals for test were guinea pigs, mice, rabbits and stone feed commonly adopted cholesterol and fat. One of the most commonly used feed formulation for guinea pigs was 90% complete feed, 1% cholesterol, 2% lard, 5% sucrose and 2% casein and after a 49-day continuous feeding, the stone formation rate was 87.40% The most commonly used formula for mice was 83.50% complete feed, 1% cholesterol, 15% fat, and 0.5% cholic acid. After a 45-day continuous feeding, the average rate of stone formation was about 89.48%. The most commonly used formula for rabbits was 98.80% complete feed, 1.2% cholesterol and after a 50-day continuous feeding, the average rate of stone formation was about 72.40%. Dogs fed with 0.3% cholesterol stone feed for 42 consecutive days showed a 100% stone formation rate. Tree shrews fed with 20% cholesterol, 20% sucrose, 15% lard (lard), 20% egg, 25% corn flour for 42 days also showed a 100% rate of stone formation while the hamster glucose fed with 74.3% casein, 20%, salt 5%, vitamin 0.5% and 0.2% choline chloride lithogenic diet for 21 consecutive days showed a 50% stone formation rate. The indicators of the test included TC, TBIL, LDL, Ba, PL, HDL, etc and serum TC, TG, TBIL, LDL, Ba, HDL, CKK, etc. Drugs were given in the process of modeling and the commonly used drugs included chenodeoxycholic acid, ursodeoxycholic acid, and compound Chinese Medicine which contains 77 kinds of medicinal herbs such as Radix Glycyrrhizae, Radix et Rhizoma Rhei, Radix Paeoniae Alba, Herba Lysimachiae, Radix Bupleuri, Herba Artemisiae Scopariae and so on. Conclusion:Stone feed is generally used in the manufacture of cholesterol gallstone animal model, but the formulation and the rate of stone formation often vary with a high cost and a time-consuming process. Traditional Chinese medicine plays a positive role in recovering the functions of hepatobiliary systems and preventing cacocholia. The evaluation indexes mainly include lipid, liver function and biliary dynamics.