To select the optimal preparation process of sugar-based kidney granules in unilateral ureteral obstruction model mice.Methods:According to macroporous resin enrichment,alcohol extraction and water extraction methods,the preparation process of Tangshen Formula I,II and III were designed.C57BL/6 mice were randomly divided into sham operation group,model group,route I,II and III groups,and positive drug fosinopril group.Six days after continuous administration,unilateral ureteral ligation was performed.Seven days after surgery,mice were given serum creatinine and urea nitrogen for 7 days.Semiquantitative analysis of renal interstitial fibrosis in each group was performed according to MASSON staining.Renal interstitial cell infiltration scores were evaluated by HE staining.Results:Each route group had a certain improvement on the pathological damage of kidney induced by UUO in mice,all of which significantly improved renal interstitial fibrosis (P<0.05).Among them,the first and third groups of the process route had better effects.The three routes can significantly improve the serum creatinine and urea nitrogen levels of mice after UUO,and significantly reduce renal interstitial cell infiltration (P<0.05).Conclusion:The preparation process of Tangshen Formula was determined as the water extraction process.