To observe the clinical effect of Duhuo Jisheng Decoction combined with methotrexate in the treatment of rheumatoid arthritis (RA) and the effects on the serum RF, ESR, CRP, TNF-α, IL-6, IL-1.Methods:A total of 92 cases with RA from Sep 2014 to Oct 2017 in Henan Xinyang Central Hospital were divided into the observation group and the control group according to random number table method, with 46 cases in each group.The control group was given methotrexate and diclofenac sodium, while the observation group was given Duhuo Jisheng Decoction based on the treatment of the control group.The course of treatment was 12 weeks.The clinical effect, the score of clinical symptom, the level of serum RF, ESR, CRP, TNF-α, IL-6, IL-1 of the two groups were observed.Results:Compared with the control group after treatment, the swelling index, morning stiffness time, 20 m walking time, pressure index, HAQ scores were decreased obviously, and the both hands grip was increased significantly.There were statistically significant differences two groups (P<0.05).The levels of serum RF, ESR, CRP, TNF-α, IL-6, IL-1 of observation group were lower than the control group (P<0.05).The total effective rate of the control group and the observation group were 73.91% and 91.30% respectively, and there were statistically significant of two groups (P<0.05).Conclusion:Duhuo Jisheng Decoction combined with methotrexate can improve the score of clinical symptom, reduce the level of serum CRP, ESR, RF, TNF-α, IL-6, IL-1, thus improve the clinical curative effect of rheumatoid arthritis.It is worthy of the clinical using.