To investigate the anti-tumor effect and mechanisms of Rui Zhen which contains hawthorn, hippophae, medlar, cordycepin, cordyceps polysaccharide, β-glucan and lycopeneon.Methods:MTT assay was used to analyze anti-proliferation activity of Rui Zhen in vitro.Anti-tumor activity and the effect of survival time were observed by mouse Lewis xenograft model in vivo.The effect of Rui Zhen on apoptosis was measured by FCM analysis.Western blot was performed to investigate the expression level of proteins in tumor tissues treated with Rui Zhen.Results:Rui Zhen has a good proliferation inhibitory activity on lung cancer cells.The IC50 values of A549 cell line, NCI-H460 cell line and NCI-H1975 cell line after 120 h were 98.42 μg/mL, 60.02 μg/mL and 60.41 μg/mL respectively.Rui Zhen can also significantly inhibit the growth of Lewis lung cancer in mice, and the growth inhibition rate of 9.0 g/kg ruthenium on Lewis lung cancer in mice was 70.0%, and it can enhance the purple pigment.The anti-tumor activity increased both the thymus index and the number of red blood cells and hemoglobin in the peripheral blood.Rui Zhen can prolong the survival time of Lewis lung cancer mice.Rui Zhen can induce apoptosis of lung cancer cells A549 and NCI-H460.At 96.0 μg/mL and 192.0 μg/mL, the number of lung cancer cells passing through the reconstituted basement membrane was significantly inhibited.Western blot results showed that ruthenium can inhibit Raf/MEK/ERK, FAK/p38 and other signaling pathways.Conclusion:Rui Zhen has certain anti-lung cancer activity in vitro and in vivo, and its mechanism may be related to blocking Raf/MEK/ERK signal transduction pathway.It is expected to develop into a natural pharmaceutical preparation for lung cancer treatment.