To investigate the immune inducing activity of Jisheng Ruanhua Decoction on mice with osteosarcoma and to provide references for synergetic treatment of tumor with traditional Chinese Medicine.Methods:Kunming mice were randomly divided into model administration group, model group, blank model group and normal group.The model administration group received intraperitoneal injection of 25mg/kg cisplatin and intragastric administration of 13mg/kg Jisheng Ruanhua Decoction, and the model group was administrated the same dosages of cisplatin and saline with the blank model group and the normal group being given the same dosage of saline respectively.After the end of the treatment course, the body weight, approximate volume of tumor, organs index and T cell subgroups were statistically analyzed.Results:Compared with the model group, Jisheng Ruanhua Decoction could significantly increase the body weight and the percentage of CD3+ T cell, CD4+ T cell, CD8+ T cell and CD4+/CD8+ ratio of the mice with osteosarcoma (P<0.05).Furthmore, Jisheng Ruanhua Decoction reduced the volume of the tumor and improved the index of thymus or spleen with no remarkable changes.Conclusion:Jisheng Ruanhua Decoction could regulate and induce immune function of mice with osteosarcoma.The combination treatment of chemotherapy and traditional Chinese medicine enhanced the immune function and inhibited tumor growth.