To study the effects of Yanlu Rukang Capsule on estrogen and progesterone in serum of rats with hyperplasia of mammary glands. Methods:All 60 Wister rats were randomly divided into six groups: blank control group, tamoxifen group, Yanlu Rukang group, Rupixiao group, disease model group, ovariectomized group. Then, the content of estradiol (E2) and progesterone (P) in serum of rats were detected by ELISA methods. Results:Compared with Rupixiao group and model group, the content of E2 in blood in the Yanlu Rukang group was significantly decreased, and the content of P was significantly higher (P<0.01); Compared with tamoxifen group, the content of E2 and P in the blood in the Yanlu Rukang group did not significantly changed, and there was no statistical significance (P>0.05). Conclusion:Yanlu Rukang capsule can significantly reduce estrogen and elevated progesterone levels in blood.