To select the optimized plan of ultrasonic assisted extracting total flavonoids in Herba Dianthi from Shaanxi by the response surface method.Methods:In this experiment, Shaanxi producing Herba Dianthi was taken as raw material, and ethanol as extraction agent.On the basis of single factor experiment, the extraction process of total flavonoids from Herba Dianthi of Shaanxi by ultrasonic assisted method was optimized with the response surface method.Results:The ultrasonic assisted extraction conditions of total flavonoids in Herba Dianthi from Shaanxi were optimized:liquid and material ratio was 10 times, extraction power 140W, 80% ethanol concentration, and the extraction rate of flavonoids could reach 0.851%.Conclusion:The process of the experimental values are basically consistent with the predicted value, indicating using response surface methodology to optimize ultrasonic assisted extraction technology of flavonoids in Herba Dianthi in Shaanxi Province is stable and feasible, and providing reference for the development and utilization of Herba dianthi resources in Shaanxi Province.