To evaluate the relationship between arrival of qi and pressure pain threshold (PPT) on Fengchi (GB 20) under different body conditions. Methods:A total of 60 subjects in our hospital from September 2017 to November 2017 were enrolled according to the inclusion and exclusion criteria, of whom 30 were healthy people and 30 were neck pain patients. All the subjects according to the enrolled order accepted one acupuncture treatment by the same acupuncturist and completed the arrival of qi evaluation. The PPT on Fengchi (GB 20) of the subjects was measured prior to the acupuncture treatment by using a SLY-HFM hand-held pain measuring instrument. Results:The PPT on Fengchi (GB 20) in patient with neck pain was lower than that in healthy people (P<0.05). There were gender differences in pressure pain threshold on Fengchi (GB 20) in the health group, and the pressure pain threshold of female was lower than male (P<0.05). There were gender differences in the pain feeling in arrival of qi in the neck pain patients, and the female were more likely to suffer from pains (P<0.05). Conclusion:Different body conditions present different PPT on specific acupoint, which the PPT of patients was lower. There were gender differences on patients' pains and the PPT, but there was no correlation between pains and the PPT.