To study the regulation effects of electroacupuncture on endogenous cannabinoid and its catabolic enzyme activity in migraine rats. Methods:Adult male SD rats were randomly divided into 4 groups：control group, model group, single acupoint group and false acupoint group. Rat jugular vein serum was taken on the 9th day of the experiment, and AEA, 2-AG, MAGL, FAAH were determined by enzyme-linked immunosorbent assay. Results:Compared with the model group, the content of AEA in the single acupoint group was increased (P<0.05) and the content of MAGL was decreased (P<0.05). Compared with the control group, the content of AEA in the model group decreased (P<0.05), and the content of MAGL in the model group increased (P<0.05). Compared with the sham acupoints group, the content of AEA in the single acupoint group was increased (P<0.05) and the content of MAGL was decreased in the single acupoint group (P<0.05). The content of FAAH and 2-AG in serum of rats in each group had no significant changes. Conclusion:It suggests that the endogenous cannabinoids and their degrading enzymes have a regulatory effect on the migraine rat model.