To observe the curative effect of “Loosing in Treating Pain” technique on chronic soft tissue damage to the waist, and evaluate its impact on peripheral blood RhoA/ROCK signal pathway.Methods:A total of 70 patients with chronic soft tissue injury in the lumbar region in our hospital from January 2013 to December 2016 were divided into the control group and the treatment group (35 cases in each group).The control group was treated with compound chlorzoxazone and functional exercise, and the observation group was added “Loosing in Treating Pain” technique and Chinese medicine of softening tendon and tonifying spleen.Two groups were received continuous 14 d intervention after the end of treatment.The simple McGill pain score, Oswestry disability index, Hamilton anxiety scale (HAMA) score and Hamilton Depression Scale (HAMD) score and lumbar muscle surface electromyography signal changes and the changes of RhoA/ROCK signal pathway concentration scales of two groups of patients were compared.Results:1) After treatment, the McGill pain score and Oswestry disability index of the two groups decreased to different degrees, and the difference was statistically significant compared with that before treatment (P<0.05), and the trend of the treatment group decreased more than the control group.The difference was statistically significant (P<0.05).2) The negative emotion of the two groups was obviously improved, and the treatment group was better than the control group (P<0.05).3) After treatment, the average electromyographic value (AEMG) of the erector and multi split muscles in the two groups were all increased.The average power frequency (MPF) was lower than that before treatment.The difference was statistically significant (P<0.05), and the improvement rate in the treatment group was significantly higher than that in the control group (P<0.05).4) ELISA results showed that the concentrations of RhoA and ROCK of peripheral blood mononuclear cells in two groups decreased after treatment.The difference between the treatment group and the control group was statistically significant (P<0.05).Conclusion:The “Loosing in Treating Pain” technique can significantly improve the clinical symptoms of chronic soft tissue injury, and its mechanism may be mediated by RhoA/Rho signal transduction pathway.