To explore the efficacy of bacillus fermentation processing Chinese medicine Flos Carthami in enhancing thrombolytic effect. Methods:Numerical system and system of numerical control technology were used for reasonable proper optimization of fermentation processing conditions. The carageen glue method was carried out on mice tail thrombosis experiment to test the fibrinolytic and blood coagulation index of rats. Results:Under the same dose (1 000 U/mL), A red hair group (Flos Carthami and C2-13 fermentation group) significantly shorten thrombus length than the rest of the groups (P<0.05). The effective extension of thrombin time (TT), prothrombin time (PT) and activated clotting time (APTT) live enzymes were delayed, and significantly shorten euglobulin dissolution time (ELT) effect (P<0.05). Conclusion:Flos Carthami by C2-13 strains fermentation processing after anticoagulant effect, can effectively improve fibrinolytic activity, and increase the efficacy of dissolving thrombus.