To investigate the effects of Yiqi Zhuyu formula Shenyuan Dan (SYD) on myocardium injury biomarkers，cardiac function，miR-24/Bim pathway protein expression in myocardial infarction rats.Methods:Fifty SD rats were randomly divided into five groups namely sham group，model group，high-，middle-，and low-dose SYD group，with 10 rats in each group.The rat myocardium ischemia model was established by ligating of anterior descending branch of coronary artery.The rats in treatment groups were performed with intragastric administration of SYD for 2 weeks.After the treatment，serum levels of creatine kinases (CK)，lactate dehydrogenase (LDH)，and cardiac function were measured.miR-24 mRNA expression was measured by RT-PCR.Bim，Bcl-2，Bax and caspase-9 protein expression in ischemic myocardium were measured by Western-blot.Results:At the end of the treatment，the serum levels of CK，LDH were significantly decreased in SYD (high，middle and low-dose) groups compared with those in model group (P<0.05，P<0.01).The cardiac function was also improved with the LVEDD and LVESD significantly decreased (P<0.05，P<0.01)，and the LVFS and EF significantly increased (P<0.05，P<0.01).The expression of miR-24 mRNA and Bcl-2 protein in ischemic myocardium were significantly increased and the Bim，Bax，caspase-9 protein were significantly decreased in SYD groups compared with those in model group (P<0.05，P<0.01).Conclusion:Yiqi Zhuyu formula SYD could relieve myocardial injury and the possible mechanism may be related to adjusting miR-24/Bim signal pathway and its related protein expression in ischemic myocardium.