To explore the clinical efficacy of puerarin injection in the treatment of diabetic peripheral neuropathy (DPN) and the influence on electromyogram and hemorheology.Methods:A total of 96 cases of DPN patients from November 2014 to February 2017 were selected and randomly divided into observation group (n＝48) and control group (n＝48).The control group was treated by α-lipoic acid with limited diet and hypoglycemic agents by or administration.The observation group was treated with puerarin injection on the basis of treatment in the control group.Both groups were treated for 2 months continuously.Comparison was made on the clinical efficacy，the symptom scores of peripheral neuropathy (TSS) and the change of electromyogram and hemorheology in 2 groups before and after treatment.Results:The total effective rate was 91.67% in observation group，which was significantly higher than 81.25% in control group (P<0.05).Compared with the scores before the treatment，the TSS scores including numbness，tingling，cramps，weakness，and the HSV，LSV，PSV，HCT as well as platelet aggregation rate of 2 groups were significantly decreased after the treatment (P<0.05 or P<0.01).And the observation group was evidently lower than the control group (P<0.01).Compared with statics before the treatment，the MNCV，SNCV of median nerve and common peroneal nerve of 2 groups were obviously increased after the treatment，and the observation group was evidently higher than the control group (P<0.01).Conclusion:In the treatment of DPN，puerarin injection can significantly relief the clinical symptoms of patients，improve the electromyogram and hemorheology，and the clinical efficacy is better than western medicine alone.