芪连扶正胶囊干预肺癌干细胞白细胞介素-10、转化生长因子-β1表达对免疫重塑的影响

doi:10.3969/j.issn.1673-7202.2018.08.037

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芪连扶正胶囊干预肺癌干细胞白细胞介素-10、转化生长因子-β1表达对免疫重塑的影响
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                        10.3969/j.issn.1673-7202.2018.08.037
                    
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基金项目:山东省自然科学基金资助项目（ZR2014HL063）

Effects of Qilian Fuzheng Capsule on the Immune Remodeling by Interfering with the Expression of IL-10 and TGF-Beta 1 in Lung Cancer Stem Cells

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目的：观察芪连扶正胶囊干预肺癌干细胞免疫相关因子白细胞介素-10(IL-10）、转化生长因子-β1（TGF-β1）表达对其免疫重塑的影响。方法：选取体外常规培养肺癌A549细胞，免疫磁珠法分选干细胞，制备芪连扶正胶囊含药血清，按实验分组干预，免疫酶联吸附试验法检测各组IL-10、TGF-β1的表达，RT-PCR法检测各组IL-10 mRNA、TGF-β1 mRNA的表达。结果：对照组、芪连扶正胶囊组、顺铂组、芪连扶正胶囊+顺铂组的IL-10表达依次为(119.03±6.99)%，(108.91±8.07)%，(96.71±5.78)%，(56.41±7.95)%；TGF-β1表达依次为(174.79±7.95)%，(160.51±8.03)%，(141.28±6.97)%，(97.34±7.63)%；IL-10mRNA表达依次为(1.06±0.19)%，(0.85±0.18)%，(0.67±0.16)%，(0.47±0.12)%；TGF-β1mRNA表达(1.27±0.26)%，(1.01±0.20)%，(0.77±0.14)%，(0.50±0.10)%。结论：芪连扶正胶囊可降低肺癌干细胞IL-10、TGF-β1表达，改善细胞免疫抑制状态，调节免疫重塑，进而抑制肺癌转移。

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To observe the effects of Qilian Fuzheng Capsule on the immune remodeling by interfering with the expression of IL-10 and TGF-β1 in lung cancer stem cells.Methods:We cultured the lung cancer A549 cells in vitro,sorted the stem cells by immunomagnetic beads,and prepared the serum containing of Qilian Fuzheng Capsule.According to the experimental group intervention,the expression of IL-10 and TGF-β1 in each group was detected by Elisa,and the expressions of IL-10mRNA,TGF-and 1mRNA in each group were detected by RT-PCR.Results:In the control group,Qilian Fuzheng Capsule group,cisplatin group,Qilian Fuzheng capsule+cisplatin group,IL-10 expression levels were (119.03±6.99)%,(108.91±8.07)%,(96.71±5.78)%,(56.41±7.95)%; TGF-β1 expression levels were (174.79±7.95)%,(160.51±8.03)%,(141.28±6.97)%,(97.34±7.63)%; IL-10mRNA expression levels were (1.06±0.19)%,(0.85±0.18)%,(0.67±0.16)%,(0.47±0.12)%; TGF-β1mRNA expression levels were (1.27±0.26)%,(1.01±0.20)%,(0.77±0.14)%,(0.50±0.10)%.Conclusion:Qilian Fuzheng Capsule can reduce the expression of IL-10 and TGF-β1 in lung cancer stem cells,improve the cellular immune suppression status,regulate immune remodeling and inhibit lung cancer metastasis.

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李慧杰,齐元富,李秀荣,张盈盈.芪连扶正胶囊干预肺癌干细胞白细胞介素-10、转化生长因子-β1表达对免疫重塑的影响[J].世界中医药,2018,(08).

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收稿日期:2017-10-29
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在线发布日期: 2018-09-10
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