To discuss protective effects of ginsenoside 20 (s)-Rg3 on renal toxicity induced by cisplatin in llc-pk1 cells. Methods:This article studied the protective effect of fermentation of black ginseng cells (FBG) and its active component ginsenoside 20 (S)-Rg3 on porcine kidney (LLC-PK1) cells in cisplatin (chemotherapy)-induced injury based on kidney protection test. Results:The cisplatin induced cell viability decreased, and then FBG was used to extract and ginsenoside 20 (S)-Rg3 was restored by FBG. After the dose dependent or extract ginsenoside 20 (S) after-Rg3 treatment may reduce cisplatin induced increased phosphorylation of c-Jun and N-terminal kinase (JNK), p53 and caspase cleavage the increase of-3 protein. By using FBG and 20 ginsenosides (S) together with-Rg3, the percentage of cisplatin induced apoptosis in LLC-PK1 cells leaded to increased significantly. Conclusion:FBG and its major ginsenoside 20(S)-Rg3, ameliorated cisplatin-induced nephrotoxicity in LLC-PK1 cells by blocking the JNKep-53-ecaspase-3 signaling cascade.