To study the anti-inflammatory and analgesic activities of different fractions extracted from Clematis manshurica Rupr.Methods:The parts of petroleum ether，chloroform，ethyl acetate and n-butanol were obtained by abstracting，extracting and concentrating.The experiment was divided into three groups：negative control group，positive control group and experimental group.In the study，mouse acetic acid writhing and lipopolysaccharide (LPS) induced RAW264.7 cell model were used to evaluate analgesic and anti-inflammatory activity of the four fractions.Results:In the experiment of analgesic，the inhibition rate of writhing was the most significant in the ethyl acetate fraction.The results of anti-inflammatory experiment suggested that the ethyl acetate fraction showed significant inhibitory effect on nitric oxide (NO).Conclusion:The anti-inflammatory and analgesic effects of different parts of Clematis mandshurica were different，and ethyl acetate fraction showed the most significant anti-inflammatory and analgesic activity.