To investigate the effects of Quyu Huatan Decoction combined with ovulation promotion scheme on proteins related to insulin signaling pathway in patients with polycystic ovarian syndrome (PCOS).Methods:A total of 100 PCOS patients in Affiliated Hospital of Qinghai University from April 2016 to April 2018 were divided into two groups (control group and observation group) by random number table,with 50 cases in each group.The control group was given clomiphene citrate (CC) and human menopausal gonadotropin (hMG) ovulation promotion scheme,while the observation group was treated with Quyu Huatan Decoction based on the same treatment of the control group.The clinical efficacy,symptom scores of Tradition Chinese Medicine (TCM),expression of proteins related to insulin signaling pathway were compared between the two groups.Results:After treatment,the total effective rate of the observation group was higher than that of the control group (P<0.05).The TCM symptom scores of two groups were compared with those before treatment,and there was no statistical difference (P>0.05).Compared with before treatment,the TCM symptom scores of two groups decreased after treatment,and the score of control group was higher than that of the observation group (P<0.05).After treatment,the AKT protein expression levels of two groups increased compared with those before treatment,and the improvement of AKT protein expression in the observation group was better than that in the control group (P<0.05).After treatment,the P-INSR,INSR,P-IRS,IRS,P-PI3K,PI3K,P-AKT,P-ERK1,ERK1,P-ERK2,ERK2 protein expression levels in two groups decreased compared with those before treatment,and the improvement of above-mentioned indexes in the observation group was better than that in the control group (P<0.05).Conclusion:Quyu Huatan Decoction combined with western medicine in treatment of PCOS is effective,which can improve clinical symptoms and regulate the expression of proteins related to insulin signaling pathway.