To explore the anti-inflammatory effects of geniposide,a main component of Fructus Gardeniae,by comparing the intervention in aqueous extract of gardenoside,the standard product of geniposide and geniposide-knockout liquid on LPS-stimulated macrophage.Methods:Geniposide aqueous extract,geniposide standard product and 50%-geniposide-knockout liquid were used to detect macrophage cytotoxicity.The control group,LPS model group,and aqueous extract of geniposide liquid group,geniposide standard product group,50%-geniposide-knockout liquid group and 50%-geniposide-backfill liquid group were set up separately.The levels of NO,IL-6 and TNF-α in each group were detected in LPS-stimulated macrophages.Results:NO release was increased in LPS-stimulated macrophages (P<10-4),and aqueous extract geniposide,geniposide standard product,and geniposide-knockout liquid reduced the release of NO.Among them,the effects of geniposide aqueous extract,50%-geniposide-knockout liquid and 50%-geniposide-backfill liquid were the strongest (P<10-2),and there was no significant difference.The effects of geniposide standard product was relatively small (P<0.05).After stimulated by LPS,macrophages secreted more TNF-α and IL-6 than the control group (P<10-4),while geniposide aqueous extract,geniposide standard product,50%-geniposide-knockout liquid and 50%-geniposide aqueous extract could reduce the secretion of cytokines (P<0.05).Conclusion:Geniposide and other components of gardenia have a certain anti-inflammatory effect,and the anti-inflammatory effects of the various components have different emphases.