To extract and determine the content of total flavonoids in Plantago asiatica L.in Panzhihua.Methods:The flavonoids extracted from Plantago asiatica L.by ultrasonic ethanol extraction and the optimal experimental conditions of the ethanol extraction extracted flavonoids from Plantago asiatica L.were obtained by the orthogonal experiment.The determination of the content of total flavanonids had been characterized by ultraviolet spectrophotometry.Results:The optimum conditions of extracting total flavonoids from Plantago asiatica L.were as follows:the solvent was 70% ethanol for 36 h, the optimum time was 35 min.The results showed the content determination of total flavonoids in Plantago asiatica L.1.53% and the average recoveries of Plantago asiatica L.roots and foliage was 98.95% and 98.18%, respectively.Under the same condition, the content of flavonoids in Plantago asiatica L.root was bigger than that in Plantago asiatica L.foliage.Conclusion:The method without any pollution, simple and rapid, provides a theoretical basis for further development and research foundation of Plantago asiatica L.