To screen the preparation technology of qi reinforcement and blood circulation promotion prescription-Kang Liu Wan (KLW) for medical institutes.Methods:A sufficient amount of C6 mice neuroglioma cell were injected into nude mice right armpit to establish a Nude Mice Tumor-Bearing model.Experimental mice were randomly divided into groups based on model tumor size and intragastrically administered with equal amounts of KLW1-4.Temozolomide was set as a positive control and vehicle (deionized water) as a model control.The nude mice weight and tumor size were measured,and the symptoms of mice was observed during the whole experiment.After 15 day's experiment,the tumor was weighed.Results:The tumor weight of KLW-1 middle and high dose group were lower than those in the model control group,and the difference was statistically significant (P<0.05).The tumor weight and tumor size in the positive group were lower than those in the model control group,and the difference was statistically significant (P<0.01).Compared with control group,all other prescription groups had no significant difference in the weight and size of tumor,and the difference was not statistically significant (P>0.05).And all preparation technology test groups had no statistically significant difference in weight (P>0.05) when compared with control group.The positive control group was significantly decreased when compared with control group after treatment,and the difference was statistically significant (P<0.01).A small number of nude mice died in each group.Conclusion:KLW has a specific anti-tumor effect in Nude Mice Tumor-Bearing model.Compared with preparation test technology,traditional prescription process technology has advantage in anti-tumor effect,prescription cost and mass-production technologies adaptability