To observe the clinical effects of activating blood circulation to resolve blood stasis and glucosamine hydrochloride capsules therapy on post-traumatic knee osteoarthritis treatment,to measure the expression of matrix metalloproteinase in post-traumatic knee osteoarthritis patients before and after treatment,and to explore its relevant molecular mechanisms.Methods:A total of 60 patients with post-traumatic knee osteoarthritis in early stage in our hospital from January 2015 to December 2017 were selected and randomly divided into the treatment group(n=30)and the control group(n=30).The control group was given oral administration of glucosamine hydrochloride capsules for 6 courses of treatment.The treatment group was using the activating blood circulation to resolve blood stasis therapy for 6 courses of treatment.The recovery of traumatic knee osteoarthritis and the symptom of traditional Chinese Medicine(TCM)in 2 groups was compared before and after treatment.The symptoms and the quantitative assessment rating scale of knee osteoarthritis,the pain index of knee and the TCM symptom scores were recorded in both groups.And the synovial fluid and serum of patients were collected to detect the expression of matrix metalloproteinase.Results:The symptoms and the quantitative assessment rating scale of knee osteoarthritis,the pain index of knee and TCM symptom scores of the 2 groups in the 1st week,the 12th week and the 24th week after treatment were significantly lower than those before treatment,and the difference was statistically significant(P<0.05).Two therapeutic methods had certain therapeutic effects on the recovery from traumatic knee osteoarthritis,and could significantly improve the TCM syndrome.There were better therapeutic effects on oral decoction of traditional Chinese medicine than oral glycosaminoglycans hydrochloride capsules on treating traumatic knee osteogenesis(P<0.05).The expression level of MMP3 was decreased significantly in both groups(P<0.0001),while the expression level of MMP13 had no significant change in both groups(P>0.05).The expression level of MMP1 was decreased significantly in the treatment group(P<0.0001),but there was no significant change in the control group after treatment(P>0.05).Conclusion:Two treatment methods have been effective in this clinical study.We found that the expression level of MMP-3 was decreased significantly after treatment in 2 therapies,which can be clinically applied.