To explore the effects of Huangqi Injection on bone marrow system and tumor markers in patients with middle-advanced stage non-small-cell lung cancer (NSCLC) after chemotherapy.Methods:A total of 75 patients with middle-advanced stage NSCLC admitted to The 180th Hospital of PLA from February 2016 to April 2017 were selected and divided randomly into control group (n=37) and observation group (n=38) according to random number table method.The patients of 2 groups received chemotherapy for four 21-day courses.The observation group was given intravenous injection of Huangqi Injection at the first 15 days of each chemotherapy course (1 time/d)，and it was continuously treated for 4 courses.The whole blood T cell subsets，bone marrow inhibition and serum tumor markers levels were detected before and after chemotherapy; the clinical efficacy in 2 groups were statistically analyzed after 4 courses of chemotherapy.Results:Compared with before chemotherapy，the whole blood CD3+，CD4+ ratio in 2 groups and CD4+/CD8+ in observation group showed a trend of decreasing first and then increasing after 1-4 courses of chemotherapy (P<0.05 or P<0.01); and observation group was significantly higher than control group after 2-4 courses of chemotherapy (P<0.01); the whole blood CD8+ ratio in the two groups showed a trend of increasing first and then decreasing (P<0.01)，and observation group was significantly lower than control group after 2-4 courses of chemotherapy (P<0.01).Compared with before chemotherapy，the serum leukocyte count，platelet count，hemoglobin level and neutrophil count in 2 groups after 4 courses of chemotherapy significantly decreased (P<0.05 or P<0.01)，and observation group was significantly lower than control group (P<0.01).Compared with before chemotherapy，serum CEA，CA-50 and CYFRA21-1 levels in 2 groups after 4 courses of chemotherapy significantly decreased，and observation group was significantly lower than control group (P<0.01); ORR in observation group was 71.05% after 4 courses of chemotherapy，which was significantly higher than that 40.54% in control group (P<0.05).Conclusion:Huangqi Injection can significantly improve the immune status and bone marrow system of patients with middle-advanced stage NSCLC after chemotherapy，reduce the levels of tumor markers，and improve the efficacy of chemotherapy.