To observe the effects of Qianliening Capsules (QC) on the extracellular matrix (ECM) via MMP-2 in treating benign prostatic hyperplasia (BPH)，and to explore the therapeutic mechanism of QC on BPH.Methods:Sprague-Dawley rats were randomly divided into blank group，BPH model group，QC low，middle，and high treatment groups.BPH rat model was established and intervened by QC.The MMP-2，LN，FN，Collagen IV in serum were examined by ELISA.BPH-1 cells without MMP-2 stimulators and BPH-1 cells added with MMP-2 stimulators were cultured respectively and treated by QC.The cell morphology and viability were observed by light microscope and MTT.The mRNA and protein expression of MMP-2，LN，Collagen IV and FN were detected by Q-PCR and Western blot assays (no MMP-2)，LN，Collagen IV and FN were detected by Q-PCR and Western blot assays (MMP-2 was added).Results:The levels of Collagen IV，FN，LN in serum of BPH rats serum treated with QC groups were reduced significantly and MMP-2 was significantly increased (P﹤0.05 or P﹤0.01).The mRNA and protein expression of LN，FN，Collagen IV in BPH-1 cells QC-treated groups were decreased significantly，and the result was more obvious in the MMP-2 added groups，but the MMP-2 was enhanced in BPH-1 cells QC-treated groups，with significant difference.Conclusion:QC has significant therapeutic effects on BPH.QC can inhibit the expression of FN，Collagen IV and LN，and enhance MMP-2，and MMP-2 can regulate FN，Collagen IV and LN，QC regulate the expression of ECM via MMP-2 is one of the mechanisms by which QC treats BPH.