To analyze the effective components of Rhizoma Coptidis and Fructus Evodiae compatibility by ultra-high performance liquid chromatography(UPLC).Methods:A total of 4 pairs of different origin of the compatibility of Rhizoma Coptidis and Fructus Evodiae.ACQUITY UPLC BEH C18 was used as a column of this study(1.7 μm,100 mm×2.1 mm).The column temperature was 40 ℃.2 μl L 10 ℃ after samples with acetonitrile(mobile phase A)-water with 0.05 % formic acid(mobile phase B)was added.The flow rate was 0.4 mL/min by gradient elution.UPLC-mass spectrometry(MS)fingerprint analysis method was established.Results:1)In the combination of Rhizoma Coptidis and Fructus Evodiae compatibility,control fingerprint and sample fingerprint were compared according to the relative retention time calibration of 16 more characteristic common peaks.The 16 peaks were respectively compared with the fingerprint of Rhizoma Coptidis characteristic peaks and Fructus Evodiae fingerprint characteristic peaks.Rhizoma Coptidis and Fructus Evodiae shared 9,15,and 16 characteristic peaks.The 1,2,13,14 common peaks were presented in Fructus Evodiae characteristic peaks.The rest peaks was presented in Rhizoma Coptidis.2)Effective components of the combination of Rhizoma Coptidis and Fructus Evodiae were qualitatively analyzed using UPLC and mass spectrometry analysis.Rhizoma Coptidis and Fructus Evodiae shared 9,15 and 16 common characteristic peaks were showed in Table 2.Magnoflorine Greenland,coptisine and 1-methyl nonyl-2-4(1H)-Quirrell ketone compounds were inferred with mass spectrometry and compounds cracking law.Conclusion:The effective components of Rhizoma Coptidis and Fructus Evodiae compatibility was established by UPLC-MS technology,providing reference for effective components and quality evaluation of Rhizoma Coptidis and Fructus Evodiae.