To optimize the extraction process of Zicao cream. Methods:The contents of total pigment of hydroxynaphthalene were determined by spectrophotometry, and hydroxysafflor yellow A was determined by HPLC. The contents of total pigment of hydroxynaphthalene and hydroxysafflor yellow A were used as the indices for evaluation. Orthogonal test was applied to optimize the extraction process. Results:The extraction process of Arnebiae Radix and Angelicae Dahuricae Radix were found to be soaked with 95% alcohol for 48 h using coarse powders, and then percolated with 10 times amount of 95% alcohol; The extraction process of Carthami Flos and Angelicae Sinensis Radix were found to be soaked with water for 30 min,and then extracted by warm maceration at 70 ℃ with 12 times amount of water at the first time and 10 times amount of water at the second time, 60 min each time. Conclusion:The process is reasonable and feasible, and lays a foundation for the formation technology of Zicao cream and its quality standards.