Glucolipid metabolic Disease (GLMD) is one of leading chronic diseases results in serious problems in public health worldwide. A growing body of evidence strongly indicate the close association between the pathogenesis of GLMD and the altered composition and function of intestinal microbiota. However，it is difficult to dissect the causative relationship between GLMD and intestinal microbiota in humans via clinical study. Therefore，the transplantation of human donor feces microbiota to animal models has been recognized as one of key approaches to reveal the cause-effect relationship between intervention therapies of Chinese Medicine or clinical characteristics and altered intestinal microbiota. This approach includes four major steps. Firstly，the intestinal microbiota should be isolated from human feces samples to prepare feces microbiota solution. Secondly，conditional germ-free mice will be induced by oral gavage of broad-spectrum antibiotic. Thirdly，conditional sterile mice was transplanted into specific bacterial species identified by whole flora or by metagenomic analysis，then was induced by animal models of metabolic diseases. Fourthly，the glucose and lipid metabolism phenotypes of the transplanted human intestinal flora were compared between the intervention group and the control group. As one of the frontier methods in the research of intestinal flora，the transplantation of human fecal microflora to animal models is of significant importance to the exploration of the mechanism underlying the therapeutic effect of Chinese medicine on GLMD.