To analyze the changes in plasma metabolism using gas chromatography-mass spectrometry (GC-MS) technology of aP2-nSREBP-1c mice with lipoatrophy. Study the differential metabolites and metabolic pathways that are closely related to lipoatrophic and explore its molecular mechanisms. Methods:Plasma samples of 6 wild-type mice in the in the control group and 6 transgene-type mice in the model group were detected using GC-MS. The result was analyzed by using multivariate statistical analysis，the metabolites were screened out which have significant difference. Results:Compared with the control group (wild-type，WT)，the plasma levels of D-(-)-Lactic acid，L-Valine，(R)-3-Hydroxybutyric acid，Urea，d-Galactose，D-Allose，Stearic acid，Palmitic acid，Myo-Inositol，Oleic acid，11-Octadecenoic acid in the model group (transgene-type，TG) increased. Pathway enrichment analysis displayed that these metabolites were mainly involved in 3 metabolic pathways which were closely related to lipoatrophy. Conclusion:Through plasma metabonomics analysis of aP2-nSREBP-1c rats，metabolic information highly associate with lipoatrophy. The finding is helpful to provide a basis for understanding the molecular mechanisms of the disease.