To establish the HPLC method for determining the contents of 6,7-dimethoxycoumarin，atractylenolide III and atractylenolide I in Bawei Yinzhu Granules. Methods:The DIKMA Diamonsil C18 (2) (4.6 mm×250 mm，5 μm) column was adopted to separate the sample at the column temperature of 30 ℃. The detection wavelength was set between 200 nm to 400 nm. The detection wavelength of 6,7-dimethoxycoumarin，atractylenolide III and atractylenolide I were 340 nm，220 nm and 275 nm respectively. The injection volume was 5 μL. The mobile phase consisted of water-acetonitrile with a gradient elution at a flow rate of 1.0 mL/min. Results:The calibration curves of were linear in the ranges of 0.1-100.0 μg/mL for 6,7-dimethoxycoumarin (R2=1.0000); the calibration curves were linear in the ranges of 0.1-100.0 μg/mL for atractylenolide III (R2=1.0000); and the calibration curves were linear in the ranges of 0.1-100.0 μg/mL for atractylenolide I (R2＝1.0000). The precision，accuracy and recovery met the determination requirements. Conclusion:The method is simple and feasible，and thus can be used for the quality control of Bawei Yinzhu Granules.