To establish quality control standards of lupus mixture. Methods:Herba Epimedii，Caulis Spatholobi，Radix Astragali seu Hedysari，Rhizoma Atractylodis Macrocephalae of lupus mixture were identified by TLC (Thin-Layer Chromatography). Gentiopicroside of lupus mixture was determined by HPLC (High Performance Liquid Chromatography). Results:Characteristics of TLC spots were clear with better separation，and negative control had no interference. It was linear in the range of 0.202 5-3.24 μg，and the rate of average recovery was 102.56% with RSD of 1.57％(n=6) for Gentiopicroside. Average concentrations of Gentiopicroside in three batches of Lupus mixture were 1.70 mg/mL. Conclusion:The method is accurate，delicacy and reproducible，providing a basis for quality control of lupus mixture.