To observe the therapeutic effect of Wumei Pills on gastrointestinal infection model mice，and to explore the effects of Wumei Pills on plasma Th1，Th2 lymphocyte factors and gastrointestinal transport function in mice. Methods:A total of 30 Kunming mice were randomly divided into blank group，model group and control group，with 10 rats in each group. The mice in the model group and the control group were given pathogenic Escherichia coli (EPEC) to simulate infectious diarrhea. The blank group was given the same dose of normal saline. After the model was successfully prepared，the control group was given the water-soluble alkaloids of Wumei Pills daily，and the dosage was converted according to the equivalent dose of human/mouse weight. The blank group and the model group were given the same dosage of saline，and the intervention lasted for 10 days. The gastric emptying rate，intestinal propulsion rate and the expression of Th1 and Th2 lymphocyte factors in peripheral blood were compared among the three groups. Results:1) The WBC and the percentage of neutrophils in peripheral blood of model group and control group were significantly higher than those of blank group (P<0.05)，and the WBC and the percentage of neutrophils in control group were lower than those of model group (P<0.05). There was no significant difference in lymphocyte percentage between 3 groups (P>0.05). 2) The gastric emptying rate and small intestinal propulsion rate of the model group and the control group were significantly higher than those of the blank group (P<0.05). The gastric emptying rate and the small intestinal propulsion rate of the control group were significantly higher than those of the model group (P<0.05). 3) The ratio of Th2 cells，IL-6，IL-10 and IL-4 in peripheral blood of model group and control group were significantly higher than that of blank group，but the level of experimental group was significantly lower than that of model group (P<0.05). The ratio of Th1 cells，the expression of IL-2，TNF-α and IFN-γ in model group and control group were significantly higher than those of blank group，but the level of model group was lower than that of model group (P<0.05). There was no significant difference between group and experimental group (P>0.05). Conclusion:Wumei Pills can effectively improve the gastrointestinal transport function in mice with gastrointestinal infection，and its mechanism may be mainly mediated by humoral immunity.