To study the clinical effects of Banxia Baizhu Tianma Decoction combined with acupuncture for the treatment of meridian headache and its influence on serological indexes. Methods:From December 2016 to December 2017，89 patients who received the treatment of headache in Nanjing Integrated Traditional Chinese and Western Medicine Hospital were selected retrospectively and were randomly divided into the observation group (n=45) and the study group (n=44). The observation group was treated with Banxia Baizhu Tianma Decoction combined with acupuncture while the control group was treated with acupuncture alone. The clinical efficacy and changes of serological indexes before and after treatment were compared between groups. Results:1) Determination of serum NO and prostaglandin:after treatment in the observation group，NO was (104.65±12.41) μmol/L，PGF2α was (27.13±9.59）pg/mL，PGE2 was (32.66±5.48）pg/mL and PGF2α /PGE2 was (0.85±0.22). In control group NO was (92.39±13.85) μmol/L，PGF2α was (33.84±10.51) pg/mL，PGE2 was (27.78±3.45) pg/mL and PGF2α /PGE2 was (1.17±0.43). 2) Plasma level:after treatment in observation group，AVP was (173.36±15.35) pg/mL，fibrinogen (3.04±0.28) g/L and ET-1 was (85.94±13.27) PG/mL. After the treatment in control group，AVP was (182.18±22.45) PG/mL，fibrinogen was (3.84±0.39) g/L and ET-1 was (102.81±16.02) PG/mL. 3) TCM syndromes scores:the total score before and after treatment in observation group were (15.95±0.38) and (3.84±0.24) respectively. In control group，the total score before and after treatment were (15.79±0.42) and (6.85±0.37) respectively. 4) The pain index of observation group and control group:after treatment in observation group，the pain degree improvement value was (1.57±0.68) and pain time improvement value was (1.56±0.59). In control group，the pain degree improvement value was (1.10±0.45) and pain time improvement value was (1.08±0.29). 5) After the treatment，of the 46 patients in observation group，2 cases were ineffective，and the total effective rate was 84.15%. In control group，of the 44 patients，11 cases were ineffective，and the total effective rate was 75%. Conclusion:The clinical effect of Banxia Baizhu Tianma Decoction combined with acupuncture is remarkable for the treatment of meridian headache，and it can improve the content of serum NO and PGE2，reduce the probability of coagulation，and prevent the headache caused by cerebral blood circulation obstruction.